Metabolic Profiling of Hematopoietic Stem Cells in Clonal Hematopoiesis (CHIP)
Recruiting · NCT05246813 · Observational (researchers observe without assigning treatment) · Lead sponsor: Imelda Hospital, Bonheiden
View the official record on ClinicalTrials.gov →Interventions studied
Blood and bone marrow cell collection
What this trial is about
Bone marrow samples will be collected from patients undergoing hip arthroplasty surgery. Blood and bone marrow samples will be used for metabolic profiling and analysis of relevant CHIP mutations. Combined single-cell transcriptomics and mutation-specific single-cell genotyping (biotin-PCR using mutation-targeted primers followed by sequencing) will subsequently be performed. The gene expression profile of wildtype and mutant hematopoietic stem cells will be compared, performing both broad gene set enrichment analysis and targeted analysis of metabolic pathways.
Who can take part
Age range
55 Years and older
Sex
All (male and female)
Healthy volunteers
No - a diagnosis or condition is required
Phase
Not specified
Study type
Observational (researchers observe without assigning treatment)
Inclusion criteria
- Age 65 years and older
Exclusion criteria
- known haematological condition (myelodysplasia, leukemia, cancer)
- inability to provide written informed consent
Where it is running
2 locations listed.
- Imelda Hospital - Bonheiden, België, Belgium
- Imelda Hospital - Bonheiden, België, Belgium